Akta fplc system operators manual marshall scientific by. A pressure sensor connected to pump module a and b. Disassemble pump cylinder and examine the piston spring according to instructions in the pump p 900 user manual. Ge p900 p900 akta fplc pump 100ml heads unit2 here is a ge p900 akta fplc pump 100ml headspredecessor to. This high flow rate is necessary when working with. Ensure your akta system is operating at peakperformance by calibrating the pressure offset. Ge amersham akta explorer 100 air fplc unit2 quality used. Ensure your akta system is operating at peakperformance by priming the system and. P901 standalone or system mounted used as standalone, or in akta explorer 100. Akta upc900 measuring instruments pdf manual download. Weve tried powering off the akta system, as well as shutting down the pc, but our cu950 is unable to connect to the akta system for us to connect using the unicorn software.
Aktapurifiertm core systems fig 1 are versatile, modular. System pump model with four pump heads in two pump modules. It is important not to proceed until all stated conditions are met and clearly understood. Aktapurifier upc 10 cytiva, formerly ge healthcare life. Powers on, digital screen and knobs respond well to input. Akta pure cytiva, formerly ge healthcare life sciences. If damaged, replace the piston according to instructions in the pump p 900 user manual. Ge p900 akta fplc pump pred 901 100ml heads here is a ge p900 akta fplc pump pred 901 100ml headspredecessor to. Amersham biosciences ge healthcare akta fplc system inquire about availability system includes phc 900 detector, uv 900 detector, p 900 pump, frac 950 fraction collector, 3 pv908 rotary valves, inv907 injection valve, m925 mixer, p 950 pump, air 900 air sensor. Akta fplc system operators manual marshall scientific. Pump p 900 manually priming buffer inlet lines open d r a w a i r o u t o f b u f f e r l i n e manual flowpath a1 or a2 buffervalvea manual flowpath b1 or b2 buffervalveb pump a pump b common inlet a2 a1 common inlet b2 b1. Feb 09, 2016 akta pump p 900 user manual readdownload box 900.
Our systems can be customized to include almost any configuration of autosamplers, detectors and pumps. Akta pure 25 purification system is intended for microgramscale protein purifications in tricorn columns up to milligramscale using hitrap and hiscreen columns. Pump parts 25 rough pumps 12 turbo pumps 6 acquity parts 27. Supply voltage to upc900, frac900 and external units. I tried purging the pump multiple times but no success. Pump p 900 manually priming buffer inlet lines open dr a w air o ut of b uff er li n e manual flowpath a1 or a2 buffervalvea manual flowpath b1 or b2 buffervalveb pump a pump b common inlet a2 a1 common inlet b2 b1. In standalone configurations, setup parameters are stated in a setup menu, see reference information b. Always keep the he inlet above the solution or close the valve. Sep 04, 20 comes with the cu950 interface, and the akta stack has the following components. The front panel displays of monitor upc900 and pump p920 can be set to show. Pulse compensation p920 0 1 pump p920 user manual 18112554 edition ad 1 introduction 1.
Bulkhead ports at rear for air, water, drain and test ports. Standard description notes harmonizedwith 2004108ec emcemissionsandimmunityrequirementsforelectricalequipmentfor measurement,controlandlaboratory use. Aktaexplorer 100 air cytiva formerly ge healthcare life. Some of our leading manufacturers include agilent, hp, waters, ge, amersham, dionex and shimazdzu. Akta laboratoryscale chromatography systems handbook. Our hplc systems such as the agilent 1100 and 1200 series, akta fplcs, and. Flow rate up to 10 mlmin, pressureup to 25mpa wavelength 190 to 700 nm p 900 pump, uv 900 detector, phc 900 phconductivity detector, frac 900 fraction collector conductivity up to mscm optional air, sample pump, autosampler, and frac950. Akta chromatography systems have been a staple in protein purification for the past two decades and used throughout the globe.
Industrial and laboratory equipment ebay shop add to favorites for any additional details, photos or expedited delivery please call or click ask a question at the bottomleft of listing descriptionhere is a ge amersham akta explorer 100 fplc pred avant unit5predecessor to. The green mark will change into the a2 and b2 side 9 repeat step 8 with the next 3 valves. Comes with the cu950 interface, and the akta stack has the following components. Wash system pump open system control and select manual.
Ge fplc aktapurifier p900 phc900 uv900 cv950 pv908. Manual flowpath b1 or b2 buffervalveb pump a pump b common inlet a2 a1 common inlet b2 b1. The termination plug is connected to the last module in the chain. Ge amersham akta explorer 100 fplc system asa global medical. We offer an assortment of refurbished and used liquid chromatography solutions ranging from hplc, lcms to fplc. The uninet 1 link connects, in series, the pc with pump p920, monitor upc900 and the frac900. Ge akta pure operating instructions manual pdf download. This ge p900 akta fplc pump is in good working order.
Flowpath buffervalvea a1 or a2 flowpath buffervalveb b1 or b2 for each line you will use draw air from all 4 ports pump a pump b common inlet a2 a1 common inlet b2 b1 4 ports. Uninet 1 link connects, in series, the pc with pump p920, monitor. Pump p900 manually priming buffer inlet lines open d r a w a i r o u t o f b u f f e r l i n e manual flowpath a1 or a2 buffervalvea manual flowpath b1 or b2 buffervalveb pump a pump b common inlet a2 a1 common inlet b2 b1. The aktaexplorer flash purification workstation passed all tests and was found to be fully functional. Ge fplc akta purifier p 900 phc 900 uv 900 cv950 pv908 inv907 iv908 7168 r in good cosmetic condition. Complete ge healthcare akta purifier 10 system from 12007. Pump parts 25 rough pumps 12 turbo pumps 6 acquity parts 27 alliance parts 35 autosampler parts 21 autosamplers 11 beckman 2 controllers 3. Also i purge d the pump with 100% methanol but everytime i purge i see air bubbles coming out of it. Upc 900 p 920 inv907 m925 new listing fully tested pharmacia hiload pump p 50.
The akta purifier 100 comes preprogrammed with application protocols and method templates, making it a fast and easy start to. Disassemble pump cylinder and examine the piston spring according to instructions in the pump p900 user manual. Box 900 solvent storage tray phc 900 ph and conductivity detector uv. The mixer volume is best changed for different flow rates. Ge amersham akta explorer 100 air fplc unit2 here is a ge amersham akta. Each pump wash requires approximately 50 ml buffer. System pump model with four pump heads in two pump modules m925 mixer. Ge healthcare pharmacia biotech akta fplc p960 sample pump 2.
It also features a tunable detector, allowing for selection of a wide spectral range. Flow rate up to 10 mlmin, pressureup to 25mpa wavelength 190 to 700 nm p900 pump, uv900 detector, phc900 phconductivity detector, frac900 fraction collector conductivity up to mscm optional air, sample pump, autosampler, and frac950. Aktafplc is a fully automated liquid chromatography system designed. Ge fplc aktapurifier p900 phc900 uv900 cv950 pv908 inv. Ge p900 akta fplc pump pred 901 100ml heads quality used. Akta explorer 100 p900 pump, uv900 monitor, and phc900 monitor cu950 communication box.
Amersham biosciences ge healthcare akta fplc system inquire about availability system includes phc900 detector, uv900 detector, p900 pump, frac 950 fraction collector, 3 pv908 rotary valves, inv907 injection valve, m925 mixer, p950 pump, air900 air sensor. Water pump backwash lines wash out wash in buffer in buffer out high pressure piston seal. Ge p900 akta fplc pump unit3 10ml heads quality used. Upc900 p920 inv907 m925 new listing fully tested pharmacia hiload pump p50. Default lines pump a a1 pump b b1 any one buffer passes through both pump heads. Tested operation of pressure sensor in p900 and p950 leak tested p900 and p950 tested uv900 monitor with nal test solution.
Ge fplc akta purifier p900 phc900 uv900 cv950 pv908 inv907 iv908 7168 r in good cosmetic condition. Step action make sure that the piece of waste tubing connected to the injection valve. Ge amersham akta explorer 100 fplc chromatography systems are made for fast development and optimization of almost any biomolecular purification, utilizing one working platform for all samples and techniques. This set up creates a lower flow rate but at a higher pressure required. Pump p900, pump p905 and pump p920 user manuals detailed system. This fplc has a capacity of up to 100 ml per minute. Box900, upc900 monitor, p900 pump, cu950 interface equipped with. Mark the instruction pump wash explorer and select appropriate pump inlets e. Connecting to supply voltage pc and a termination plug. Pump p901, pump p903, pump p905 and pump p manualzz. Optional components for the aktafplc system are described in aktafplc optional configurations user manual. View and download amersham akta upc900 user manual online.
Filter, regulator, pump lubricator, air shutoff valves. Ge amersham akta explorer 100 fplc pred avant unit6. All eluents can be mixed with a high degree of accuracy an reproducibility. Manually tested frac950 positions, flow, and drop sync, all of which were. Ge healthcare pharmacia biotech akta fplc p960 sample. The ge akta purifier 10 is a flexible modular fast protein liquid chromatography system. In this automatic program, the pump is washed for 4 minutes with mq pumped from inlet a11. Free download akta fplc p920 manual programs rutrackerroof. Inlet or outlet check valves not functioning correctly.
Ge p900 p900 akta fplc pump 100ml heads unit2 quality. The ge akta purifier 100 is a fast protein liquid chromatograph or fplc, which is capable of handling a wide range of wavelengths. Aktaexplorer am ers ha m bio scie nce am er sham bio ci n a m e r s h a m. Purge system pump b follow the instruction below to purge both pump heads of system pump b. Box 900 solvent storage tray phc 900 ph and conductivity detector uv 900 detector p 900 pump with 100 ml heads. Air driven lubricated piston pump capable or testing up to 30,000 psi.
When purging a buffer line both pump heads must be purged. If damaged, replace the piston according to instructions in the pump p900 user manual. Ge akta fplc explorer 10 system manual marshall scientific. Box 900, upc 900 monitor, p 900 pump, cu950 interface equipped with.
Amersham biosciences ge healthcare akta fplc system. The figure below shows the purging parts for the pump p900. Used amersham pharmacia akta explorer 18111241 liquid. Amersham pharmacia akta explorer 100 fplc system at.
For fast protein liquid chromatography of tens of grams of protein, akta pure 150 can be used with flow rates up to 150 mlmin. This ge p 900 akta fplc pump is in good working order. The pump can be used to form accurate and reproducible gradients. Ge fplc manual akta fplc explorer 100 operators manual marshall scientific ge healthcare. Purification can be performed at microgram, milligram or gram scale, depending on the core system and kits selected.
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